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REDETECT THE EXPRESSION OF VECTOR pcDNA-CA JEMBRANA VIRUS USING POLYMERASE CHAIN REACTION METHOD WITH AMPLIKON 650 bp
This research used polymerase chain reaction method to redetect the gag-ca gene in plasmid recombinant pcDNA-CA vector. In this research is done analysis repeats towards inserted gag-ca gene of jembrana disease virus in plasmid pcDNA that made by Kusumawati, et al., in 2002. Plasmid that transformated in E coli DH5α with luria bertani (LB) media then isolated by using high pure plasmid isolation kit. The isolated plamid recombinant pcDNA-CA that isolated then amplified with ready to go pcr using primary specific. The specific primer recognize sequence of gag-ca gene in 650 bp lenght. The identification uses electroforesis 1% gel agrose with run 90 volt/ cm gel. The result shows band dna that bring gag-ca gene after PCR reside in 650 base pair (bp). It could mean that plasmid with insert of gag-ca gen still can be isolated and positive in test after kept for long time.
Keywords: jembrana virus, gag-ca gene, polymerase chain reaction
